PhytophthoraV1, Main, Exploration, bibRecord, 000956

Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.

Identifieur interne : 000956 ( Main/Exploration ); précédent : 000955; suivant : 000957

Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.

Auteurs : Yufeng Fang [États-Unis] ; Brett M. Tyler [États-Unis]

Source :

Molecular microbiology [ 1365-2958 ] ; 2017.

RBID : pubmed:28213898

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Motifs (genetics), Amino Acid Sequence (genetics), Basic-Leucine Zipper Transcription Factors (metabolism), Cell Nucleus (metabolism), Molecular Sequence Data (MeSH), Nuclear Localization Signals (genetics), Nuclear Localization Signals (metabolism), Phytophthora (genetics), Phytophthora (metabolism), Protein Domains (genetics), Sequence Homology, Amino Acid (MeSH), Transcription Factors (metabolism).
MESH :
- chemical , genetics : Nuclear Localization Signals.
- chemical , metabolism : Basic-Leucine Zipper Transcription Factors, Nuclear Localization Signals, Transcription Factors.
- genetics : Amino Acid Motifs, Amino Acid Sequence, Phytophthora, Protein Domains.
- metabolism : Cell Nucleus, Phytophthora.
- Molecular Sequence Data, Sequence Homology, Amino Acid.

Abstract

Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.

DOI: 10.1111/mmi.13652
PubMed: 28213898

Affiliations:

États-Unis

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Le document en format XML

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<term>Cell Nucleus (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Nuclear Localization Signals (genetics)</term>
<term>Nuclear Localization Signals (metabolism)</term>
<term>Phytophthora (genetics)</term>
<term>Phytophthora (metabolism)</term>
<term>Protein Domains (genetics)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
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<term>Données de séquences moléculaires (MeSH)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines (métabolisme)</term>
<term>Motifs d'acides aminés (génétique)</term>
<term>Noyau de la cellule (métabolisme)</term>
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<term>Séquence d'acides aminés (génétique)</term>
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<term>Signaux de localisation nucléaire</term>
<term>Séquence d'acides aminés</term>
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<term>Facteurs de transcription à motif basique et à glissière à leucines</term>
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<term>Signaux de localisation nucléaire</term>
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<term>Sequence Homology, Amino Acid</term>
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<front><div type="abstract" xml:lang="en">Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.</div>
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<CopyrightInformation>© 2017 John Wiley & Sons Ltd.</CopyrightInformation>
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Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.

Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki